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Anuj Sharma

Plant Pathologist

Currently, I am a Postdoctoral Research Associate at University of Florida. I work with Jeff Jones and Erica Goss in Department of Plant Pathology. My current research focuses on bacterial spot of tomato and pepper caused by several species of Xanthomonas. Among other things, I am working on mapping resistance genes, developing bacterial dispersal models and understanding mutation rate of bacterial effectors.

Email:anujsharma@ufl.edu

Education

PhD in Plant Pathology 2021

University of Florida, Department of Plant Pathology

BS in Agriculture 2016

Tribhuvan University, Institute of Agriculture and Animal Science

Awards

APS Foundation Student Travel Award2020

American Phytopathology Society

IFAS Travel Award2020

University of Florida, Institute of Food and Agricultural Sciences

PPGSO Travel Award2019

Plant Pathology Graduate Student Organization @ UF

Graduate School Preeminence Fellowship2017

University of Florida, Department of Plant Pathology

Merit Scholarship2013

Tribhuvan University, Institute of Agriculture and Animal Science

Skills

Molecular

High Resolution Melting curve qPCR Topo-cloning Gateway cloning Protein fusions Confocal microscopy RNAi gene silencing CRISPR editing SnapGene Geneious

Bioinformatics

Sequence alignment De novo assembly Genome annotation Bulk segregant analysis SV  discovery CNV  discovery Biomarker discovery Comparative Genomics DE analysis PE analysis Phylogenetics BioPython

Programming languages

R Julia Python Shell/Bash Powershell HTML CSS Javascript Markdown React AutoHotKey

Other softwares

Ms Office Trello Teams Photoshop Inkscape Git/Github Docker VS Code Jupyter Fusion360 Netlify ArcGIS

Research experience

Postdoctoral Research Associate 2021 – Present

University of Florida, Department of Plant Pathology

Fine-mapping of bs8 resistance in pepper.

Estimating bacterial mutation rate over host growing season.

Understanding fitness cost of a bacteriocin gene.

Graduate Research Assistant 2017 – 2021

University of Florida, Department of Plant Pathology

Effect of XopJ2 in Xanthomonas perforans ingress and egress.

Understanding transcriptomic changes in citrus due to Xanthomonas citri infection.

Study of mutation rate of Xanthomonas TAL effectors.

Modeling the dispersal of Xanthomonas perforans in field and greenhouse.

Understanding the role effector XopJ2 in spread of Xanthomonas perforans.

Mapping of novel bacterial spot resistance genes in pepper.

Research Intern 2016 – 2017

Nepal Agriculture Research Council, Horticulture Research Station

National coordinated varietal trials of several vegetable crops.

Evaluation of various coffee rust biocontrol strategies.

Resistance breeding against late blight resistance in tomato.

Undergrad Research Assistant 2015 – 2016

Tribhuvan University, Plant Pathology Department

Screening finger millet accessions for blast resistance.

Disease diagnosis for plant diagnostic clinic.

Teaching experience

University of Florida

Guest Lecture (Spatio-temporal Dispersal Modeling) in Leveraging AI in Plant Pathology2021
Guest Lecture (Epidemiology) in Bacterial Plant Pathogens2021
Guest Lecture (Dissemination, Survival and Ingress) in Bacterial Plant Pathogens2021
Guest Lecture (Stramenopiles) in Fungal Plant Pathogens2020
Teaching Assistant for Fungal Plant Pathogens2020
Guest Lecture (Identification of Bacteria) in Bacterial Plant Pathogens2020
Teaching Assistant for Bacterial Plant Pathogens2020
Teaching Assistant for General Plant Pathogen2019

Nepal Agriculture Research Council

Course supervisor for JTA work experience program2017

Affiliations

American Phytopathological Society

Designer, Graduate Student Arts committee2019 – 2020
Member2017 – Present

UF Plant Pathology Graduate Student Organization

Treasurer2019 – 2020
Member, Outreach committee2017 – 2021
Member2017 – 2021

Agri-Youth Nepal

Founding Member2014 – 2016

Projects

Mutation rate of TAL effectors

Contents are not disclosed yet.

Modeling dispersal of Xanthomonas perforans

Models were developed to predict the movement of Xanthomons perforans in the field and in green house based on weather condition and other factors. Of special interest was a type-3 effector gene XopJ2 which seemed to provide fitness for dispersal. Effect of the gene was accessed in field studies and the mechanism of fitness is being studies in green house.

Mapping of resistance genes in pepper

Resistance genes against bacterial spot of pepper in PI163192 and UENF1381 were mapped. F2 population was developed by crossing with ECW variety. QTLs were mapped by bulked sequence analysis of whole genome sequences of resistance and susceptible bulks. HRM curve and CAPS marker were developed at target region and further fine-mapping was performed in backcross F2 populations.

Rust management strategy in organix coffee

23 coffee varieties from previous year’s multi-season trial were scored for incidence and severity of coffee leaf rust. Three varieties 'Catimor', 'Ketisic' and 'San Roman' performed best against coffee rust. Increased shading, increased spacing and removal of crop debris reduced incidence. Triadimefon was effective as control agent whereas the effect of Verticillium lecanii was inconsistent.

Breeding tomato for late blight tolerance

NARC HRD breeding lines 'HRD-7' and 'HRD-20', which showed tolerance to late blight, were crossed with other breeding lines to develop hybrid lines with late blight tolerance. The lines were evaluated in field trials against commercial variety 'Shrijana'. Three hybrids (HRA-14 X HRD-7, HRA-20 X HRD-1, HRA-20 X HRD-2) showed high tolerance and were recommended for multi-location CVT.

Screening finger millet for blast resistance

50 finger millet accession from National Agriculture Genetic Resources Center were screened for resistance against blast cause by Magnaporthe grisea. Plants were scored for disease severity and AUDPC and DMRT was used for accessing level of resistance. 5 accessions showed moderate quantitative resistance and were recommended for further screening.

Publications

Research papers

2021

Sharma A, Ference CF, Shantharaj D, Baldwin EA, Manthey JA, and Jones JB. Transcriptomic analysis of changes in Citrus × microcarpa gene expression post Xanthomonas citri subsp. citri infection. European Journal of Plant Pathology 162:163–181.

Sharma A, Timilsina S, Abrahamian P, Minsavage GV, Colee J, Ojiambo PS, Goss E, Vallad GE, and Jones JB. Need for speed: Bacterial effector XopJ2 is associated with increased dispersal velocity of Xanthomonas perforans. Environmental Microbiology 23:5850-5865.

2020

Abrahamian P, Sharma A, Jones JB, and Vallad GE. 2020. Dynamics and spread of bacterial spot epidemics in tomato transplants grown for field production. Plant Disease 105(3):566-575.

2017

Bhatta A, Sharma A, et al. 2017. Resistant and susceptible response of finger millet to seedling blast (Pyricularia grisea SACC.). The International Journal of Information Research and Review 4(12):4804-4809.

In Preparation

Sharma A, Minsavage GV, Gill US, Hutton SF, and Jones JB. Identification and mapping of bs8, a novel locus conferring resistance to bacterial spot caused by Xanthomonas gardneri. Phytopathology.

Jibrin MO, Sharma A, Goss EM et al. Intercontinental dissemination of tomato pathogen Xanthomonas gardneri during the expansion of global trade in hybrid seeds.

Review papers

2021

Osdaghi E, Jones JB, Sharma A, et al. A Centenary for Bacterial Spot of Tomato and Pepper. Molecular Plant Pathology. 22(12):1500-1519.

2019

Sharma A, Jones JB, and White FF. Recent advances in developing disease resistance in plants. F1000Research 2019, 8(F1000 Faculty Rev):1934.

In Preparation

Sharma A, Abrahamian P, Carvalho R, Choudhary M, Paret ML, Vallad GE, and Jones JB. Future of Bacterial Disease Management in Crop Production. Annual Review of Phytopathology.

Dickstein ER, Sharma A, Jones JB, et al. Recovery Plan for Ralstonia solanacearum Race 3 Biovar 2.

Book chapters

In preparation

Sharma A, Timilsina S and Jones JB. Initial identification of common bacterial genera. In Laboratory Guide for Identification of Plant Pathogenic Bacteria, 4th Edition. American Phytopathological Society (APS Press).

Ham JH, Sharma A, Jones JB and Chun W. Burkholderia and Robbsia. In Laboratory Guide for Identification of Plant Pathogenic Bacteria, 4th Edition. American Phytopathological Society (APS Press).

Sharma A and Jones JB. Bacterial Spot. In Compendium of Pepper Diseases, 2nd Edition. American Phytopathological Society (APS Press).

Conference presentations

APS Plant Health 20192019

Role of AvrBsT in dispersal of Xanthomonas perforans and severity of bacterial spot of tomato

16th Biennial FPS Meeting2019

Characterization of bacterial spot resistance gene against Xanthomonas gardneri in Hungarian pepper

Posters

APS Plant Health 20202020

Genetic mapping of bacterial spot resistance gene against Xanthomonas gardneri in Hungarian pepper

References

Dr. Jeffrey B. Jonesjbjones@ufl.edu

Distinguished professor, Department of Plant Pathology, University of Florida

Dr. Samuel F. Huttonsfhutton@ufl.edu

Associate professor, Gulf Coast Research and Education Center, University of Florida

Dr. Gary E. Valladgvallad@ufl.edu

Associate professor, Gulf Coast Research and Education Center, University of Florida

Dr. Erica M. Gossemgoss@ufl.edu

Assistant professor, Department of Plant Pathology, University of Florida

Contact

Address

Work:
2500 Hull Rd, Fifield Hall #2307, Gainesville, FL 32611

Phone number

Office: 6464-372 (253)
Personal: 0917-312 (253)

Email address

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